Art. No.: 5121ZON
EuroProxima Zearalenone is a competitive enzyme immunoassay for quantitative analysis of zearalenone in cereals, milk, milk powder and serum.
The kit is based on a microtiter plate (12 strips, each 8 wells), precoated with sheep antibodies to rabbit IgG. In one incubation step, specific antibodies (rabbit antizearalenone), enzyme labelled zearalenone (-HRP) and zearalenone standards or samples are added to the precoated wells. The specific antibodies are bound by the immobilized sheep anti-rabbit antibodies and at the same time free zearalenone (in the standard solution or in the sample) and enzyme labelled zearalenone compete for the specific antibody binding sites (competitive enzyme immunoassay). After an incubation time of one hour, the non-bound (enzyme labelled) reagents are removed in a washing step. The amount of bound enzyme conjugate is visualized by the addition of chromogen substrate (tetramethylbenzidine, TMB). Bound enzyme transforms the chromogen into a coloured product. The substrate reaction is stopped by the addition of sulfuric acid. The colour intensity is measured photometrically at 450 nm and is inversely proportional to the zearalenone concentration in the sample.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection Limit)||Cereals (12.5 μg/kg (ppb)), milk (0.625 μg/L), milk powder (0.5 μg/kg), serum (1.25 μg/L)|
Cereals, milk, milk powder and serum.
Zearalenone in cereals, milk, milk powder and serum.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|