Qualitative detection and identification of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris (Biorad, Agilent Mx3005P™, Rotorgene™).
Alicyclobacillus is a gram-positive, strictly aerobic, acidophilic and thermophilic spoilage bacteria, especially important for the fruit juice industry, where it has been shown to produce high amounts of guaiacol imparting offflavors to fruit juices. Alicyclobacillus is of special interest to the fruit juice canning industry because common pasteurization techniques do not deactivate the spores. Therefore early screening of Alicyclobacillus is very important for the industry since contamination can lead to considerable loss of quality and subsequently to economic losses for the producers. Alicyclobacillus acidoterrestris is considered the most important spoilage species within the Alicyclobacillus genera, but other e.g. Alicyclobacillus acidocaldarius have als been isolated from spoiled products. General culture methods take 10-12 days to detect the presence of Alicyclobacillus species in samples, further 24h to confirm guaiacol production and identification of isolates require even longer. To overcome this difficulty, GEN-IAL developed a multiplex real-time PCR method to detect and differentiate Alicyclobacillus species in fruit juices and concentrates much more earlier.
To avoid false negative PCR-results an Inhibition control (ROX/DQ) is amplified together in one reaction vessel with the specific sequence. Optional: the use of Uracil-N-Glycosylase will eliminate any contamination with Uracil containing amplicons from former PCRs (the enzyme is not part of this kit).
|Art. No.||TPABM 0050|
|Test format||50 reactions|
|Sample preparation||The use of SES 0050 is recomended|