RIDASCREEN® Nitrofuran (AOZ)

Art. No.: R3703

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Product info

RIDASCREEN® Nitrofuran (AOZ)

Art. No.: R3703

Intended use

RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.

General Information

Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently used in animal production due to their excellent antibacterial and pharmacokinetic properties. They have also been used as growth promoters during the production of shrimp, poultry and pigs. Long term animal experiments have shown that the parent compounds and their metabolites have carcinogenic and mutagenic characteristics. This led to the prohibition of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also prohibited.

The analysis of nitrofurans are based on the detection of the tissue bound metabolites of nitrofurans. The parent compounds are difficult to detect accurately since they are metabolized very rapidly after treatment. The tissue bound nitrofuran metabolites however are present for a long time after administration and they are used to detect nitrofuran abuse.

Prior to analysis, the metabolites have to be derivatized by incubation with 2-Nitrobenzaldehyde into NP-AHD, NP-AMOZ, NP-AOZ and NP-SEM.


  • Sensitive: The detection limit is approx. 50 ng/kg (ppt) in shrimp, fish and milk. The detection limit is approx. 100 ng/kg in meat, liver and whole egg.
  • Rapid: Assay results within 1 h 15 minutes.
  • Simple: Test can be performed immediately.
Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.

Art. NoR3703
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each)
Sample preparationHomogenization (milk samples: precipitation), derivatization, extraction, centrifugation, evaporation and degreasing.
Incubation time1 h 15 min
LOD (Detection Limit)Shrimp, fish, milk approx. 50 ng/kg, meat, liver, whole egg approx. 100 ng/kg
Validated matrices

Shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.

Detected analyte

Nitrofuran (AOZ) in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.

Available application notes

Fish and shrimp.

EvaluationMicrotiter plate spectrophotometer (450 nm)