Art. No.: RAE8201

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Art. No.: RAE8201

Intended use

RIDASCREEN®EASY Mustard is an enzyme immunoassay for the quantitative determination of mustard.

General Information

Mustard belongs to the 14 allergens which must be listed in the EU according to Regulation (EU) No. 1169/2011 and in some other countries on food labels as a food allergy trigger.
Even small traces of allergens in foods may induce allergic reactions in sensitive people. Symptoms include skin rash, swelling, shortness of breath, asthma, stomach pain, nausea and vomiting and even anaphylactic shock. Those affected must strictly avoid all foods containing mustard proteins. Some people with a mustard allergy have a cross-allergy to other foods such as fruit or nuts.
There are considerable deficiencies in labeling and quality assurance, particularly with the allergen mustard. For example, undeclared traces of mustard are repeatedly found in food during inspections. An important source of danger is cross-contamination during cultivation, processing (dust formation during grinding) or packaging. The allergenic proteins in mustard do not lose their allergenic effect through processing. Therefore, processed samples have been investigated during assay validation of the RIDASCREEN®EASY Mustard, which were already contaminated with mustard prior processing. Hence, in this type of sample (incurred samples), the mustard proteins have passed through significant steps of the food production.

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.

Art. NoRAE8201
Test formatMicrotiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparationHomogenization and extraction
Incubation time50 min
Room temperature
LOD (Detection Limit)0 - 0.07 mg/kg (ppm) mustard protein (mean)
0.02 mg/kg mustard protein (depending on the matrix)
LOQ (Limit of quantification

0.2 mg/kg (ppm) mustard protein

Cross Reactivity.

Cross reactivity to rapeseed and fenugreek.

Validated matrices

Pesto, sausage, cream cheese, dressing, noodles and caraway.

Detected analyte

Different kinds of mustard (yellow/white, brown, black mustard).

EvaluationMicrotiter plate spectrophotometer (450 nm)