Intended use:

The real-time PCR test detects DNA of brown (Indian), yellow and black mustard according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

General information:

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg mustard / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.

Accessories:

Specifications
Art. No. S3609
Test format 100 reactions
LOD (Detection limit) ≤ 0.4 mg / kg
LOQ (Limit of quantification)

1 mg / kg using SureFood® PREP Advanced, protocol 1

Evaluation Real-time PCR instrument with two detection channels (510 nm and 580 nm)
E-mail linksales@r-biopharm.de
Files
InstructionsGerman/English
SDSerklaerung-zu-congen-msds_2018-12.pdf (German)
confirmation-congen-msds_2018-12.pdf (English)
Similar products
RIDASCREEN®FAST Mustard
RIDASCREEN®FAST Mustard
RIDASCREEN®FAST Mustard is a sandwich enzyme immunoassay for the quantitative analysis of mustard in ketchup, sausage and cream cheese.
Lateral Flow Mustard incl. Hook Line
Lateral Flow Mustard incl. ...
The Lateral Flow Mustard incl. Hook Line from Bioavid is an immunochromatographic test for the qualitative detection of mustard residues in environ...
SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC
SureFood® ALLERGEN 4plex So...
The SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific...

Start typing and press Enter to search