Intended use:

RIDASCREEN®FAST Crustacean is a sandwich enzyme immunoassay for the quantitative analysis of crustaceans which can be present as ingredient or contamination in raw or cooked food.

General information:

Shrimp, crab, prawns, krill, lobster, langouste and crayfish are described as crustaceans. Especially, the muscle protein tropomyosin can cause allergic reactions. Severe sensitizations are not rare. Allergies to crustaceans occur more frequently in adult people and remain there for life. For crustaceans, a reference dose of 10 mg crustacean-protein was determined (Vital concept). Assuming an average protein content of crustaceans of 20 %, this corresponds to 50 mg crustacean. The RIDASCREEN®FAST Crustacean 2nd generation (R7312) is thus sensitive enough to enable food allergen labeling according to the VITAL concept.
Crustaceans can be present as an ingredient or as a contamination in raw and processed products. According to the regulation (EU) No. 1169/2011, crustaceans must be declared on food labels as they can induce allergic reactions. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.

Specifications
Art. No. R7312
Test format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Sample preparation Homogenization, extraction and centrifugation
Incubation time 30 min
Room temperature
LOD (Detection limit) 2.0 mg / kg (ppm) crustacean (mean)
0.9 - 2.6 mg / kg (ppm) crustacean*
*depending on matrix
LOQ (Limit of quantification)

20 – 160 mg / kg (ppm) crustacean

Cross reactivity

Minor cross-reaction was detected with mustard, curcuma, beans, mussels and arthropods.

Available application notes
  • More sensitive analysis (improved LOQ of 5 mg / kg (ppm)).
Detected analyte

Crustacean-proteins (mainly tropomyosin)

Evaluation Microtiter plate spectrophotometer (450 nm)
E-mail linksales@r-biopharm.de
Files
InstructionsGerman/English
Italian
SDSR7312_german.zip (German)
R7312_english.zip (English)
R7312_french.zip (French)
R7312_italian.zip (Italian)
R7312_spanish.zip (Spanish)
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