Easy to use microbiological microtiter plate test for the quantification of total niacin (enriched and natural niacin) in food, feed and pharmaceutical products. All required reagents and standard are contained in the test kit.
Niacin is extracted from the sample and the extract is diluted. The niacin assay – medium and the diluted extract are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply of niacin. Following the addition of niacin as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth of Lactobacillus plantarum in relation to the extracted niacin is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Incubation time||44 - 48 h in the dark at 37 °C (98.6 °F)|