EuroProxima Deoxynivalenol
Art. No. 5121DON
Product info
Intended use
EuroProxima Deoxynivalenol is a competitive enzyme immunoassay for quantitative analysis of deoxynivalenol (DON) in cereals, feed, food, beer and silage.
General Information
Deoxynivalenol belongs to the group of trichothecenes produced essentially by a broad range of Fusarium moulds of which F. graminearum and F. culmorum are the
most important. These fungi typically develop during prolonged cool, wet growing and harvest seasons to produce Fusarium head blight in cereal crop. Deoxynivalenol, also known as ‘vomitoxin’, because of its potent emetic effects in swine, is most often associated with Fusarium head blight. Some fungal strains make DON by a 3-
acetylated precursor (mainly in Europe and Asia) and others produce a 15-acetylated precursor (mainly in North and South America). The amount of 3-acetyl-DON and/or
15-acetyl-DON in a sample is approximately 10 % of the amount of DON present. Significant concentrations of DON are frequently detected in wheat, barley, corn and
oats, while lower levels are usually found in rye, sorghum and rice. About 40-50 % of all samples analysed were found positive for DON with average contents of 290 µg/kg (range 2 – 10.000 µg/kg). This assay is suitable for screening purposes on the presence of the mycotoxin DON in food and feed products, but also in liquid samples as beer. For confirmation purposes a previous HPLC isolation in combination with massspectrometry analysis is necessary.
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/RBNL.
| Specifications | |
|---|---|
| Art. No | 5121DON |
| Test format | Microtiter plate with 96 wells (12 strips with 8 wells each). |
| Sample preparation | For mentioned matrices fast and efficient extraction methods are included in the kit description. |
| Incubation time | 1 h 30 min |
| LOD (Detection Limit) | Cereals (1.5 μg/kg (ppb)), feed (30 μg/kg), food (30 μg/kg), beer (1.5 μg/L), silage (50 μg/kg) |
| Validated matrices | Cereals, feed, food, beer and silage. |
| Detected analyte | Deoxynivalenol (DON) in cereals, feed, food, beer and silage. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Files | |
|---|---|
| Instructions | English |
| MSDS | 5121DON.zip (English) |
