The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Agilent AriaDx, R-Biopharm RIDA®CYCLER and Agilent Mx3005P.
The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90's. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (S1053) is recommended.|
|LOD (Detection limit)||The SureFood® GMO Plant PLUS PCR has a limit of detection of ≤ 5 DNA copies|