SureFood® GMO QUANT MON863 Corn

Art. No.: S2051

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SureFood® GMO QUANT MON863 Corn

Art. No.: S2051

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Intended use

SureFood® GMO QUANT MON863 Corn is a real-time PCR for the detection of the relative MON863 corn DNA amount to the total corn DNA content. Therefore, the kit contains two PCR systems, one for detection of a MON863 corn-specific gene (YieldGard™ Rootworm™ corn, OECD unique identifier MON-ØØ863-5) and one for the detection of a corn gene (reference gene).

 

The specific detection is according to the validated method of the European Commission.

General Information

The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.

Accessories

Specifications
Art. NoS2051
Test format2 x 50 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The SureFood® GMO QUANT MON863 Corn specific PCR has a limit of detection of < 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed corn grain.
LOQ (Limit of quantification

The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for MON863 corn DNA is 0.1 %.