SureFood® GMO QUANT MON863 Corn
Art. No.: S2051
SureFood® GMO QUANT MON863 Corn is a real-time PCR for the detection of the relative MON863 corn DNA amount to the total corn DNA content. Therefore, the kit contains two PCR systems, one for detection of a MON863 corn-specific gene (YieldGard™ Rootworm™ corn, OECD unique identifier MON-ØØ863-5) and one for the detection of a corn gene (reference gene).
The specific detection is according to the validated method of the European Commission.
The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.
|2 x 50 reactions
|For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
|LOD (Detection Limit)
|The SureFood® GMO QUANT MON863 Corn specific PCR has a limit of detection of < 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed corn grain.
|LOQ (Limit of quantification
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for MON863 corn DNA is 0.1 %.