SureFood® GMO QUANT T25 Corn
Art. No.: S2017

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SureFood® GMO QUANT T25 Corn

Art. No.: S2017
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Intended use

The test detects the relative quantitative T25-Corn DNA amount.

Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZMØØ3-2) and the other one specific for Corn (reference system). The event-specific detection of T25-Corn is according to the validated method of the European Commission.

 

General Information

The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Applied Biosystems 7500, Agilent Mx3005P and Agilent AriaDx.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS2017
Test format2 x 50 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The T25-Corn PCR has a limit of detection of ≤ 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed corn grain.
The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
LOQ (Limit of quantification

The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for T25-Corn DNA is 0.1 %.