RIDA®QUICK Gluten quant.
Art. No. RAL7073
Product info about RIDA®QUICK Gluten quant.
Intended use
Fastest and most simple quantitative LFD method for gluten detection!
Ensures a safe, fast and simple quantitative analysis of gluten residues from wheat, rye and barley on surfaces, in clean-in-place (CIP) water and in foods (raw and processed) in test strip format.
RIDA®QUICK Gluten quant. is an R5-based immunochromatographic test for the quantitative analysis of contaminations by prolamins from wheat (gliadins), rye (secalins), and barley (hordeins). The used specific monoclonal antibody (R5) detects the amino acid sequences in prolamins that are toxic to coeliac disease patients. However, it does not react with epitopes of prolamins from oats which are tolerable for most celiac patients.
Benefits:
- Only quantitative LFD using the R5 antibody on the market.
- Results expressed as gluten (no conversion factor needed).
- Enhanced validation: usage of incurred matrices as well as wheat, rye and barley as contaminant – meeting the latest AOAC guidelines.
- Innovative and simple test evaluation with the RIDA®SMART APP Allergen.
RIDA®SMART APP Allergen:
The RIDA®SMART APP Allergen is required to evaluate the quantitative gluten test strip (available in the Google Play Store).
General Information
The use of gluten in foodstuffs is extremely common because of its useful effects on e.g. texture, moisture retention and flavor. However, gluten intolerance disorders like coeliac disease require a permanent gluten-free diet to avoid clinical symptoms. According to the Codex Alimentarius (CODEX STAN 118-1979) foods for special dietary use for persons intolerant to gluten may contain up to 20 mg/kg gluten to be tolerated by celiac patients. Foods containing < 20 mg/kg gluten can be labelled “gluten-free”.
The threshold of 20 mg/kg has been adopted by national legislations in many countries. Gluten of wheat, rye and barley is a mixture of prolamin and glutelin proteins. Prolamins from wheat are named gliadins. The prolamine content of wheat gluten is defined as 50 % (CODEX STAN 118-1979). R5-based ELISA methods, which have been calibrated against gliadin (prolamin part of wheat gluten), therefore need to be multiplied by factor of 2 to calculate the gluten concentration. Due to the calibration against gluten, theRIDA(R)QUICK Gluten quant. test provides results directly in mg/kg gluten. Thus, the calculation factor is not needed any longer.
The official type I method for gluten determination of the Codex Alimentarius is an ELISA, which uses the R5 antibody in combination with a special extraction buffer, the Cocktail (patented) (Mendéz method). The RIDASCREEN® Gliadin ELISA (Art. No. R7001) fulfils this requirement. The RIDA®QUICK Gluten quant. test strips also use the R5 antibody and show a good correlation with the official method R5 ELISA RIDASCREEN® Gliadin. Only R-Biopharm AG is authorized to use the R5 antibody in immunochromatographic lateral flow tests.
Following extraction solutions are validated for this method:
- RIDASCREEN®EASY Extraction Tablets (Art. No. RAA0008)*
Accessories
- RIDASCREEN®EASY Extraction tablets
- Set of 3 processed Gliadin Assay Controls
- RIDA®QUICK quant. evaluation kit
- RIDA®SMART BOX
Dear customers,
we provide the documents for our products in an electronic format which include the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01. January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.
| Specifications | |
|---|---|
| Art. No | RAL7073 |
| Test format | 15 test strips |
| Incubation time | 10 min, room temperature |
| LOD (Detection Limit) | Surfaces: 0.038 µg/mL Gluten CIP water: 0.072 µg/mL Gluten Food samples: 0.700 mg/kg Gluten |
| LOQ (Limit of quantification | Surfaces: 0.2 µg/mL Gluten |
| Cross Reactivity. | No cross-reaction was detected in 106 tested foods. |
| Validated matrices | Baked goods, sauces, chocolate containing desserts, spices, rice flour Stainless steel CIP water |
| Detected analyte | • The main epitope of the R5 antibody is the amino acid sequence QQPFP which is part of many celiac-toxic sequences. |
| Evaluation | RIDA®SMART Allergen App (RSA) - available in Google Play Store |
| Files | |
|---|---|
| Instructions | German/English |
| MSDS | SDS_RAL7073-1.zip (All languages) |
Patent Marking:
The extraction tablet of the test kit contains sulfite. Food inspection methods using a sulfite containing extractant as used in this test kit and/or corresponding detection kits are subject
to the following patents of MORINAGA & Co., Ltd.: European Patent EP 2 224 239 B1, Australian Patent AU 2008 330 507 B2, United States Patent US 8 859 212 B2, Japanese Patent JP 5 451 854 B2. The patent holder has granted R-Biopharm AG a license to use the protected technology in said territories.
