Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Benefits:

  • Improve workflow using innovative product.
  • Simultaneous detection of 11 or more legislated mycotoxins.
  • Single extraction, with excellent precision and accuracy.

Accessories:

Specifications
Art. No. RBRP128/ RBRP128B
Test format RBRP128: 10 immunoaffinity columns
(3 ml format),
RBRP128B: 50 immunoaffinity columns
(3 ml format)
Sample preparation A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.
Incubation time 60 seconds
LOD (Detection limit) B1 0.05 ng/ml, B2 0.05 ng/ml,
G1 0.05 ng/ml, G2 0.05 ng/ml,
OTA 0.05 ng/ml, FB1 1 ng/ml,
FB2 1 ng/ml, DON 0.1 ng/ml,
ZON 0.05 ng/ml, T-2 0.05 ng/ml,
HT-2 0.05 ng/ml
Validated matrices

Food and feed.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Deviation from the methods described in our Instructions For Use and Application Notes may not result in optimum results. Please contact your local R-Biopharm distributor for further information. Please contact your local R-Biopharm distributor for further information.

Detected analyte

Aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in food and feed.

Evaluation It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.
E-mail linksales@r-biopharm.de
Files
InstructionsEnglish
SDS2020-04_p128_11myco-ms-prep_safety-data-sheet-v2_en_lowres.pdf (English)
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