DZT MS-PREP® immunoaffinity columns contain highly specific monoclonal antibodies directed against DON, zearalenone, T-2 and HT-2 toxin. The columns lead not only to a clean-up of the sample but also to a selective purification of the mycotoxins. After the extraction of the sample with methanol / water and subsequent filtration, the sample extract is applied to the immunoaffinity column. If the relevant mycotoxins are present in the sample, they are bound by the corresponding antibodies. The columns are washed and the mycotoxins are eluted with methanol. The collected eluate is then used for analysis with LC-MS/MS.
DON, zearalenone, T-2 and HT-2 toxin belong to the trichothecenes, which is the largest group of mycotoxin-producing Fusarium fungi. DON is also called vomitoxin because it causes nausea in pigs and other animals. In some cases, food poisoning has also been reported in humans in connection with DON-contaminated cereals. DON is often found together with zearalenone in wheat, barley and corn. Zearalenone is an estrogenic substance that can cause the corresponding symptoms in pigs, cattle, poultry and rodents. High concentrations are associated with fertility, impaired fetal development and embryonic death.
T-2 and HT-2 toxin are mycotoxins produced by different species of Fusarium. These mycotoxins are frequently found in cereals and feed and can cause acute symptoms such as nausea, feed refusal and low weight gain in livestock and poultry. However, these mycotoxins can also lead to chronic impairments in animals, such as immunosuppression, oral lesions and lethargy.
- Simultaneous detection of 4 legislated mycotoxins in a single LC-MS/MS run.
- One sample preparation and extraction required per sample for the analysis, saving time and money.
- Excellent recoveries for all toxins, complying with EU Method Performance Criteria.
|Test format||RBRP73: 10 immunoaffinity columns|
(1 ml format),
RBRP73B: 50 immunoaffinity columns
(1 ml format)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 seconds|
|LOD (Detection limit)||DON 0.1 ng/ml, ZON 0.05 ng/ml, |
T-2 0.05 ng/ml, HT-2 0.05 ng/ml
Food and feed.
|Available application notes|
Methods are available for all matrices covered by legislation as well as additional commodities. Deviation from the methods described in our Instructions For Use and Application Notes may not result in optimum results. Please contact your local R-Biopharm distributor for further information.
DON, Zearalenon, T-2 und HT-2 Toxin in food and feed.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|