The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxin M1 in milk and dairy products.
The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. The total extraction and clean-up time takes approximately 30 minutes to perform. The result is improved clean-up and concentration of the toxin from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for arge scale analysis of samples.
- AFLAPREP M WIDE is an improvement on AFLAPREP M as the wide column is easier to handle and prevents column blockage with high fat content samples.
- AFLAPREP M WIDE is available in 10 and 50 column kits to suit customers with various testing requirements.
- Additional Application Notes are available for more complex dairy products.
|Article Numbers||RBRP124 / RBRP124B|
|Test format||10 columns (3 ml format) (RBRP124), |
50 columns (3 ml format) (RBRP124B)
|Incubation time||60 sec|
|LOD (Detection limit)||M1 0.05 ng/ml (ppt)|
Milk and milkpowder including cow´s milk, goats milk, soya milk and cream.
|Available application notes|
Methods are available for all matrices covered by legislation as well as additional commodities. Deviation from the methods described in our Instructions For Use and Application Notes may not result in optimum results. Please contact your local R-Biopharm distributor for further information.
Aflatoxin M1 in milk and milkpowder including cow´s milk, goat´s milk, soya milk and cream.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|