Intended use:

Easy to use microbiological microtiter plate test for the quantification of total vitamin B2 (enriched and natural vitamin B2) in food, feed and pharmaceutical products.

General information:

Vitamin B2 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B2 assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of vitamin B2. Following the addition of vitamin B2 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B2 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).

Certificates:

Specifications
Art. No. P1007
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation For the determination of added and total vitamin B2 the sample has to be treated with enzymes and thereafter heat extracted.
Incubation time 44 - 48 h in the dark at 37 °C (98.6 °F)
Files
InstructionsGerman/English
SDSP1007_german.zip (German)
P1007_french.zip (French)
P1007_english.zip (English)
P1007_spanish.zip (Spanish)
P1007_italian.zip (Italian)
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