Easy to use microbiological microtiter plate test for the quantification of total folic acid (enriched and natural folic acid) in food, feed and pharmaceutical products.
Folic acid is extracted from the sample and the extract is diluted. The diluted extract and the folic acid assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of folic acid. Following the addition of folic acid as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted folic acid is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
- VitaFast® Folsäure/Folic Acid Dotierstandard/Spiking Standard
- Chicken Pancreatin (γ-Glutamylhydrolase)
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Incubation time||44 - 48 h in the dark at 37 °C (98.6 °F)|