Usage of Compact Dry X-SA is a simple and safe test procedure for determination and quantification of Staphylococcus aureus in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient pad.
The ready-to-use, chromogenic Compact Dry plates are suitable for in-process controls as well as controls of finished products. With the Promedia ST-25 swab tests (Art. No. Z0302, available from R-Biopharm) Compact Dry plates may also be used for surface sampling and therefore applied for quantitative hygiene monitoring even at surfaces which are difficult to reach.
Staphylococcus aureus is a ball shaped bacterium which can be observed as a grape-like clustered organism under the microscope. Cells are sized between 0.8 and 1.2 µm, Gram-positive and non-motile. S. aureus is a natural commensal of humans which lives on skin and upper airways and doesn’t cause diseases in general. In case of infections with pathogenic strains or in immunodeficient persons S. aureus is able to cause skin infections and abscesses but also life threating diseases as pneumonia, meningitis, endocarditis, or toxic shock syndrome (TSS).
One reason for the facultative pathogenicity of S. aureus is its ability to form enterotoxins (SET), which can accumulate in contaminated food and can’t be inactivated totally by heat treatment. Contaminations of food stuffs with S. aureus are almost always caused by humans (hands, sneezing, coughing). S. aureus therefore is considered as indicator for poor personal hygiene. Beside its relevance for food hygiene S. aureus also plays a significant role in hospital hygiene. Especially strains of S. aureus which have acquired resistances against common antibiotics the so called „methicillin resistant Staphylococcus aureus” (MRSA) might pose a non-assessable danger as they are difficult-to-treat.
Compact Dry X-SA detects S. aureus after only 24 h incubation time at 37 °C. The bright blue colonies are easy to identify on the nutrient pad and therefore easy to evaluate.
Compact Dry X-SA has been approved by MicroVal (certificate No. 2008LR14), NordVal (certificate No.042) and AOAC-RI (certificate No. 081001). During the according studies a broad range of different food matrices has been tested.
|Art. No.||HS9621; HS9622|
|Test format||100 nutrient plates or 40 nutrient plates, respectively - – where four plates are packed in one aluminum bag|
|Incubation time||24 ± 2 h at 37 ± 1°C|