Immunoaffinity columns for use in conjuntion with an HPLC or LC-MS/MS system for detection of biotin in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer, centrifuged and the supernatant filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the vitamin is then released following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
- AOAC Official First Action Method - 2016.02
|Art. No.||P82 / P82B|
|Test format||10 wide format immunoaffinity columns (P82).|
50 wide format immunoaffinity columns (P82B).
|Detection limit||LOD: 25 ng/ml|