A competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices.
Carazolol is a nonspecific β-adrenergic blocking agent that is used in pigs to prevent stress during transport. A less widespread use is in the prevention of reproductive stress in dairy cows. In the EU, maximum residue limits (MRLs) have been established for both pigs and cattle. The compound is listed in Annex I of Council Regulation (EEC) No. 2377/90. The marker residue is the parent substance.
The Carazolol ELISA is a competitive enzyme immunoassay based on antibodies directed against carazolol.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For urine, tissue, live and kidney fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Urine 2.2 ppb|
Tissue 0.3 ppb
Liver/kidney 3.0 ppb
Carazolol 100%, Clenbuterol <0.02%, Salbutamol <0.02%, Adrenaline <0.02%, Epinephrine <0.02%, Alprenolone <0.02%