Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices.

General Information:

Florfenicol (FF) is an effective broad-spectrum antibiotic and an analogue of chloramphenicol (CAP). Due to the ban of the use of CAP in food-producing animals, FF is used to control infections in humans, pigs, poultry, non-ruminating cattle and aquaculture. Within the EU, Maximum Residue Limits (MRLs) for FF have been established. These MRLs are described as: “Sum of florfenicol and its metabolites measured as florfenicol-amine”. For this reason, EuroProxima has developed an ELISA that is specific for the measurement of florfenicol-amine.


Article Numbers5091FLOA
Test format Microtiter plate with 96 wells (12 strips with 8 wells each)
Sample preparation For the mentioned matrices, fast and efficient methods are included in the test kit description.
Incubation time 1 h 30 min
LOD (Detection limit) Tissue 5.1 µg/kg (ppb), fish 8.3 µg/kg, kidney 11.6 µg/kg, liver 7.4 µg/kg, milk 2.1 µg/l, egg 5.0 µg/kg
Detection capability CCβ Tissue 15 µg/kg, fish 15 µg/kg, kidney 15 µg/kg, liver 15 µg/kg, milk 8 µg/l, egg 8 µg/kg
Validated matrices

Tissue, fish, kidney, liver, milk and egg.

Detected analyte Florfenicol-amine in various matrices.
Evaluation Microtiter plate spectrophotometer (450 nm)
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