Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of Florfenicol-amine in various matrices

General information:

Florfenicol (FF) is an effective broad-spectrum antibiotic and an analogue of chloramphenicol (CAP). Due to the ban of the use of CAP in food-producing animals, FF is used to control infections in humans, pigs, poultry, non-ruminating cattle and aquaculture. Within the EU, Maximum Residue Limits (MRLs) for FF have been established. These MRLs are described as: “Sum of florfenicol and its metabolites measured as florfenicol- amine”. For this reason, EuroProxima has developed an ELISA that is specific for the measurement of florfenicol-amine.

Art. No. 5091FLOA
Test format Microtiter plate, 96 wells.
Sample preparation For tissue, fish, kidney, liver, milk and egg fast and efficient methods are included in the kit manual.
Incubation time 90 min
Detection limit Tissue 5.1 ppb
Fish 8.3 ppb
Kidney 11.6 ppb
Liver 7.4 ppb
Milk 2.1 ppb
Egg 5.0 ppb
Cross reactivity

Florfenicol-amine 100%, Florfenicol <10%, Thiamphenicol <10%, Chloramphenicol <10%

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