A competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices.
Flumequine is a second-generation Quinolone antibiotic and is mainly active against Gram negative bacteria. In Europe the MRLs for Flumequine in the different matrices depend on the matrix and the species from which the matrix origins. MRLs vary from 200 μg/kg (muscle) to 1500 μg/kg (kidney). The antiserum used in the ELISA is specific for Flumequine and does not cross-react with any of the other Fluoroquinolones tested. For detection of most Fluoroquinolones in one test we advise the generic Fluoroquinolones ELISAs (5101FLUQG and 5101FLUQGII).
The Flumequine ELISA is a competitive enzyme immunoassay based on antibodies directed against flumequine.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, serum, meat, shrimp, egg, urine, feed, honey and water fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Milk 13 ppb|
Serum 1 ppb
Meat <0.1 ppb
Shrimp <0.1 ppb
Egg 3.5 ppb
Urine 5.5 ppb
Feed 10 ppb
Honey <10 ppb
Water 3 ppb
Flumequine 100%, All other fluoroquinolones tested < 0.1%