Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices.

General information:

Fluoroquinolones are a synthetic class of antibiotics which all act by inhibition of bacterial DNA-gyrase. The antiserum used in the ELISA is directed against the main Fluoroquinolones including Enrofloxacin and its metabolite Ciprofloxacin. In combination with our specific ELISA against Flumequine nearly all Fluoroquinolones can be screened for in a fast and reliable way.

The Fluoroquinolones ELISA is a competitive enzyme immunoassay based on antibodies directed against fluoroquinolones.

Art. No. 5101FLUQG
Test format Microtiter plate, 96 wells.
Sample preparation For milk, egg, tissue, cheese, honey, feed, water, serum and urine fast and efficient methods are included in the kit manual.
Incubation time 90 minutes
Detection limit Milk 3 ppb
Egg 6 ppb
Tissue and whole egg 0.5 ppb
Soft and hard cheese 6 ppb
Honey 2 ppb
Feed 5 ppb
Water 2 ppb
Tissue 0.3 ppb
Serum 2.5 ppb
Urine 7 ppb
Cross reactivity

Ciprofloxacin 124%, Norfloxacin 100%, Enrofloxacin 92%, Danofloxacin 89%, Nadifloxacin 85%, Pefloxacin 70%, Piromidic acid 62%, Oxolinic acid 57%, Enoxacin 57%, Lomefloxacin 40%, Fleroxacin 40%, Ofloxacin 18%, Marbofloxacin 16%,
Pipemidic acid 5%, Gatifloxacin 5%, Sarafloxacin 4%,
Levofloxacin 3%, Flumequine 2%, Difloxacin 1%, Pazufloxacin 1%

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