A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples.
Neomycin belongs to a group of broad spectrum antibiotics called aminoglycosides. These antibiotics are mainly used to treat infections caused by Gram negative bacteria in human and animals. All aminoglycosides are potentially toxic compounds and therefore maximum residue limits (MRLs) have been set by the European Union. In combination with the streptomycin and gentamicin ELISA EuroProxima offers a unique panel for the detection of these three aminoglycosides in a range of matrices.
The Neomycin ELISA is a competitive enzyme immunoassay based on antibodies directed against neomycin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each). |
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description. |
|Incubation time||1 h 30 min, 4 °C / RT|
|LOD (Detection limit)||Milk and milk powder 6.25 µg/l (ppb), tissue 31.25 µg/kg, honey 15.63 µg/kg, serum/ plasma 6.25 µg/l, urine 8.42 µg/l|
|Detection capability CCβ||Milk 10 ng/ml , milk powder 10 ng/g, tissue 50 ng/g, honey 25 ng/g, serum/plasma 6.25 ng/ml, urine 10 ng/ml|
Milk, milk powder, tissue, honey, serum/plasma and urine samples.
|Detected analyte||Neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples.|
|Evaluation||Microtiter plate spectrophotometer (450 nm) |