Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of ochratoxin A prior to HPLC or LC-MS/MS.
The online ochratoxin immunoaffinity cartridge is used in conjunction with the RIDA®CREST system, combining automated online sample application with quantitative analysis of ochratoxin. The immunoaffinity cartridge contains a monoclonal antibody that is specific for ochratoxin coupled to a hydrophilic polymer that can withstand high pressure. This enables the cartridge to be incorporated directly online with the HPLC system part of the RIDA®CREST system.
The immunoaffinity cartridge offers highly specific, sensitive, rapid and automated analysis for ochratoxin in a wide range of food and feed matrices. Using the ochratoxin immunoaffinity cartridge, the sample application, washing and elution is performed online for a specified number of analyses before the cartridge is automatically removed and replaced with a new cartridge. This level of reuse has been found to offer optimum cartridge performance and prevent interference or carryover.
Following extraction of the toxins from the sample with solvent, the extract is filtered, diluted and transferred to an autosampler vial. The diluted extract is injected onto the immunoaffinity cartridge and any toxin present in the sample are retained by antibody in the cartridge. Unbound material is then removed by washing the cartridge and sending the resulting wash to waste. Subsequently the toxins are released from the antibody following online elution and the complete eluate from the cartridge is quantitatively analysed for ochratoxin by HPLC or LC-MS/MS.
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