Immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of ochratoxin A in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.
|Art. No.||P14 / P14B|
|Test format||10 wide format immunoaffinity columns (P14)|
50 wide format immunoaffinity columns (P14B)
|LOD (Detection limit)||LOD: 0.05 ng/ml|
|Approvals||Used in CEN collaborative trials. Methods using these columns have been approved by the AOAC.|