Intended use:

Premi®Test is a microbial screening test for the detection of antibiotic residues in food and feed in less than 4 hours.

Premi®Test allows to screen meat (beef, pork, poultry), fish, shrimps, eggs, liver, kidney, urine and feed for the residues of β-lactams, Cephalosporines, Macrolides, Tetracyclines, Sulphonamides, Aminoglycosides, Quinolones, Amphenicoles and Polypeptides.

PremiTest is a ‘Performance Tested Method’ of the AOAC Research Institute for the screening of Penicillin in bovine meat (060601) and holds the ‘VALIDATION NF’ by AFNOR for the screening of β-lactams, Macrolides, Tetracyclines, Sulphonamides in beef, pork and poultry (RBP 31/02 – 04/11).

General Information:

Treatment of farm animals with veterinary pharmaceuticals such as antibiotics leads to drug residues in the tissues of the animals and establishes a health risk for the consumers of animal food products. Premi®Test is an easy antibiotics residue screening test which allows fast screening of food products by all members of the food production chain such as farmers, slaughterhouses, meat-processors and analytical laboratories.

The test is based on the inhibition of the growth of Bacillus stearothermophilus, a thermophilic bacterium which is very sensitive to many antibiotics and sulpha compounds. A standardised number of spores are imbedded in agar medium containing selected nutrients. When Premi®Test is heated at 64 °C, the spores will germinate. If no inhibitory substances are present the germinated spores will multiply with the production of acid. This will be visible by a colour change from purple to yellow. When anti-microbial compounds are present in sufficient amount (above the detection limit) the spores will be unable to germinate and therefore no colour change will be observed.

Art. No. R3925
Test format 25 ampoules
Sample preparation Meat (beef, pork, poultry): extraction of meat juice using a ‘meat press’ (contained in thePremi®Test Starter Kit ZPT-2000).
More sample preparation protocols are available for the analysis of antibiotic residues in fish, shrimps, eggs, liver, kidney, plasma/serum, urine from cattle/pork and feed for swine/poultry.
Incubation time Approximately 3 hours
Detection limit In line with EU Maximum Residue Limits (MRL’s).
Approvals AOAC-RI (060601)
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