Premi®Test is a microbial screening test for the detection of antibiotic residues in food and feed in less than 4 hours.
Premi®Test allows to screen meat (beef, pork, poultry), fish, shrimps, eggs, liver, kidney, urine and feed for the residues of β-lactams, Cephalosporines, Macrolides, Tetracyclines, Sulphonamides, Aminoglycosides, Quinolones, Amphenicoles and Polypeptides.
PremiTest is a ‘Performance Tested Method’ of the AOAC Research Institute for the screening of Penicillin in bovine meat (060601) and holds the ‘VALIDATION NF’ by AFNOR for the screening of β-lactams, Macrolides, Tetracyclines, Sulphonamides in beef, pork and poultry (RBP 31/02 – 04/11).
Treatment of farm animals with veterinary pharmaceuticals such as antibiotics leads to drug residues in the tissues of the animals and establishes a health risk for the consumers of animal food products. Premi®Test is an easy antibiotics residue screening test which allows fast screening of food products by all members of the food production chain such as farmers, slaughterhouses, meat-processors and analytical laboratories.
The test is based on the inhibition of the growth of Bacillus stearothermophilus, a thermophilic bacterium which is very sensitive to many antibiotics and sulpha compounds. A standardised number of spores are imbedded in agar medium containing selected nutrients. When Premi®Test is heated at 64 °C, the spores will germinate. If no inhibitory substances are present the germinated spores will multiply with the production of acid. This will be visible by a colour change from purple to yellow. When anti-microbial compounds are present in sufficient amount (above the detection limit) the spores will be unable to germinate and therefore no colour change will be observed.
|Test format||25 ampoules|
|Sample preparation||Meat (beef, pork, poultry): extraction of meat juice using a ‘meat press’ (contained in thePremi®Test Starter Kit ZPT-2000).|
More sample preparation protocols are available for the analysis of antibiotic residues in fish, shrimps, eggs, liver, kidney, plasma/serum, urine from cattle/pork and feed for swine/poultry.
|Incubation time||Approximately 3 hours|
|Detection limit||In line with EU Maximum Residue Limits (MRL’s).|
AFNOR VALIDATION NF (RBP31/02 – 04/11)
|Links||Tips and Tricks|