Premi®Test is a microbial screening test that allows the detection of antibiotic residues, especially in fresh meat (beef, pork, poultry) in less than 4 hours. The test is sensitive to antibiotics of the following groups: β-lactams, cephalosporins, macrolides, tetracyclines, sulfonamides, aminoglycosides, quinolones, amphenicols and polypeptides.
Premi®Test is a ‘Performance Tested Method’ of the AOAC Research Institute for the screening of Penicillin in bovine meat (Certificate No. 060601). Furthermore, it holds the ‘VALIDATION NF’ by AFNOR for the screening of β-lactams, macrolides, tetracyclines, sulphonamides in beef, pork and poultry (RBP 31/02 – 04/11).
Treatment of farm animals with veterinary pharmaceuticals such as antibiotics leads to drug residues in the tissues of the animals and establishes a health risk for the consumers of animal food products. Premi®Test is an easy antibiotics residue screening test which allows fast screening of food products by all members of the food production chain such as farmers, slaughterhouses, meat-processors and analytical laboratories.
Premi®Test is based on the inhibition of the growth of Bacillus stearothermophilus, a thermophilic bacterium which is very sensitive to many antibiotics and sulpha compounds. A standardised number of spores are imbedded in agar medium containing selected nutrients. When Premi®Test is heated at 64 °C, the spores will germinate. If no inhibitory substances are present the germinated spores will multiply with the production of acid. This will be visible by a colour change from purple to yellow. When anti-microbial compounds are present in sufficient amount (above the detection limit) the spores will be unable to germinate and therefore no colour change will be observed.
- AOAC-RI - 060601
- AFNOR - VALIDATION NF (RBP 31/02-04/11)
|Test format||4 x 25 ampoules|
|Sample preparation||Meat (beef, pork, poultry): extraction of meat juice using a ‘meat press’ (contained in the Premi®Test Starter Kit ZPT-2000).|
Recommendations for sample preparations for fish, shrimps, eggs, kidney, liver, urine, blood and feed are available at R-Biopharm AG.
|Incubation time||Approximately 3 hours|
|LOD (Detection limit)||In line with EU Maximum Residue Limits (MRL’s).|