Intended use:

Solid phase clean-up columns for the purification of aflatoxins and zearalenone from cereals.

General Information:

PuriTox AflaZON columns can be used for the simultaneous detection of aflatoxins and zearalenone by HPLC or GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results. The clean-up columns can be used in conjunction with HPLC for the analysis of pigmented samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column. The solid phase clean-up column is used in conjunction with HPLC or GC for the analysis of difficult samples.


  • One sample preparation and extraction required per sample for the analysis of aflatoxin and zearalenone,
    saving time and money.
  • Optimised HPLC methods for the analysis of aflatoxin in peanuts (PuriTox Aflatoxin Art. No. RBRP25) or
    aflatoxin and zearalenone in cereals (PuriTox AflaZON Art. No. TC-M160).
  • Improved clean-up of peanut and cereal samples reducing background interference, improving the chromatography
    and the accuracy of results.

Article NumbersTC-M160
Test format 25 solid phase clean-up columns (syringe format)
Sample preparation A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.
LOD (Detection limit) Total AFT 1 ng/ml, ZON 50 ng/ml
Validated matrices

Cereals and peanuts.

Detected analyte

Aflatoxins and zearalenone in cereals.

Evaluation It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.
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