Intended use:

RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.

General Information:

The main causative agent of food poisoning are enterotoxins of Staphylococcus aureus next to the intoxication with Salmonella. Among the strains of Staphylococcus aureus other Staphylococci species as S. hyicus and S. intermedius are able to produce one or more heat stable proteins which behave like enterotoxins.

Generally, it is assumed that a population of 5 x 105 cells of enterotoxin-producing Staphylococcus aureus strains per gram of food is required to lead to an intoxication. However, other studies showed that only 100 – 200 ng of Staphylococcus enterotoxins can lead to symptoms of food poisoning.

SET intoxications have been frequently associated with pasta, finished meat products, ham, pies, chicken meat products, fish, fish products, milk, milk products, ice-cream, egg products, salads, pastries and cake stuffing as well as preparations from these food products. The enterotoxins of the serological groups A, B, C, D and E are very significant.

Benefits:

  • Official European Screening Method for Staphylococcus enterotoxins.
  • Europe-wide ring study according to ISO 16140.

.

Specifications
Article NumbersR4105
Test format Microtiter plate with 96 wells (12 strips with 8 breakable wells each),
96 determinations.
Sample preparation „Official European Preparation Method“ (dialysis concentration method) or simplified method.
Incubation time 2 h 45 min
LOD (Detection limit) Simplified working up:
- Liquid samples: 0.25 ng toxin / ml sample,
- Solid samples: 0.375 ng toxin / g sample,
- Supernatants from bacterial cultures: 0.25 ng toxin / ml sample.

Dialysis concentration:
- Liquid samples: 0.05 ng toxin / ml sample,
- Solid samples: 0.05 ng toxin / g sample.
Detected analyte

Staphylococcus aureus enterotoxins A, B, C, D, E in total

Evaluation Microtiter plate spectrophotometer
Approvals Official European Screening Method for Staphylococcal enterotoxins in food.
Files
Instructions
Links Old Instructions for use
MSDS
Similar products
RIDASCREEN® SET A,B,C,D,E
RIDASCREEN® SET A,B,C,D,E
RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and soli...
SureFast® Staphylococcus aureus PLUS
SureFast® Staphylococcus au...
The SureFast® Staphylococcus aureus PLUS is a real-time PCR for the direct, qualitative detection of a specific Staphylococcus aureus DNA sequence....
QuickGEN PCR Kit Fusarium spp.
QuickGEN PCR Kit Fusarium spp.
Detection of Fusarium spp. in beverages, cereals etc. by real-time PCR.

Product support

Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success.

Start typing and press Enter to search