Intended use:

RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage.

General Information:

Cow’s milk contains 3.2 % proteins which consist of 10 % β-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen especially for children is β-lactoglobulin. For adults the caseins become more dominant. Milk can induce allergic reactions at infancy. Whey or milk powder is often added to food products, therefore it is recommended to determine β-lactoglobulin in food. The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, milk and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.

Benefits:

  • Fast: Only 30 minutes incubation time during procedure (by far the fastest compared to competitors).
  • Specificity: no cross reactivities have been observed in a wide range of matrices (85).
  • Validated for automates.

Accessories:

Specifications
Article NumbersR4912
Test format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Sample preparation Homogenization and extraction
Incubation time 30 min
Room temperature
LOD (Detection limit) 0.042 mg / kg (ppm) ß-lactoglobulin (mean)
0.024 – 0.073 mg / kg (ppm) ß-lactoglobulin*
*depending on matrix
LOQ (Limit of quantification)

0.167 mg / kg (ppm) ß-lactoglobulin

Cross reactivity

There is a cross reactivity to sheep’s, goat’s and buffalo’s milk.

Validated matrices

Rice crispies, chocolate and sausage.

Available application notes
  • Extraction for heavily liquid absorbing matrices using RIDA® Extractor 2 (R4613).
  • Sample extraction of pine nuts, sunflower and pumpkin seed.
Detected analyte β-lactoglobulin of cow’s milk
Evaluation Microtiter plate spectrophotometer (450 nm)
Files
InstructionsGerman/English
Italian
MSDSr4912_german.zip (German)
r4912_english.zip (English)
r4912_french.zip (French)
r4912_italian.zip (Italian)
r4912_spanish.zip (Spanish)
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