The SureFast® Animal+Plant Control 3plex real time PCR controls the DNA-preparation. In addition a differentiation between the DNA of vertebrates and plants is possible. The test contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC/HEX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II/Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, BMS MIC, Agilent Mx3005P and Agilent AriaDx.
|Test format||100 reactions|
Kit content: Reaction Mix, Taq Polymerase, Internal Control DNA, Positive Control
|LOD (Detection limit)||The SureFast® Animal+Plant Control 3plex real-time PCR has a limit of detection of < 500 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|