The SureFast® Emetic Bacillus cereus PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the cereulide synthetase of the emetic Bacillus cereus.
Each reaction contains an internal amplification control (PLUS).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.|
To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
|Detection limit||The SureFast® Emetic Bacillus cereus PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|