Intended use:
The SureFast® Emetic Bacillus cereus PLUS is a real-time PCR for the direct, qualitative detection of a specific cereulide synthetase DNA sequence of the emetic Bacillus cereus.
Each reaction contains an internal amplification control (IAC).
General Information:
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.
Accessories:
| Article Numbers | F5127 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended. |
| LOD (Detection limit) | The SureFast® Emetic Bacillus cereus PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Instructions |
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