The test detects the DNA of the emetic toxin of Bacillus cereus. Each reaction contains an internal amplification control (PLUS).
For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended. To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.02, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
|Test format||100 reactions|
|Detection limit||The SureFast® Emetic Bacillus cereus PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|