Intended use:

The SureFast® Emetic Bacillus cereus PLUS is a real-time PCR for the direct, qualitative detection of a specific cereulide synthetase DNA sequence of the emetic Bacillus cereus.
Each reaction contains an internal amplification control (IAC).

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.

Accessories:

Specifications
Art. No. F5127
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.
LOD (Detection limit) The SureFast® Emetic Bacillus cereus PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English
Similar products
TANBead Maelstrom™ 8 Autostage
TANBead Maelstrom™ 8 Autostage
The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation...
SureFast® Emetic Bacillus cereus PLUS
SureFast® Emetic Bacillus c...
The SureFast® Emetic Bacillus cereus PLUS is a real-time PCR for the direct, qualitative detection of a specific cereulide synthetase DNA sequence ...
Compact Dry X-BC
Compact Dry X-BC
Usage of Compact Dry X-BC is a simple and safe test procedure for determination and quantification of Bacillus cereus in foods or raw materials. Th...

Start typing and press Enter to search

qtpydb-0048_gen-ial-test