SureFast® Fecal Screen 4plex

Art. No.: F5504

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Product info

SureFast® Fecal Screen 4plex

Art. No.: F5504

Intended use

The SureFast® Fecal Screen 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Enterococci, Escherichia coli in water.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.


Art. NoF5504
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Aqua (Art. Nr. F1023) is recommended.
LOD (Detection Limit)The SureFast® Fecal Screen 4plex real-time PCR has a limit of detection of < 5 DNA copies.
Validated matrices

Water portions horse
Pond water
Stool sample in the water