Intended use:

The test detects Enterobacteriaceae DNA.

Each reaction contains an internal amplification control (IAC).

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.

Accessories:

Specifications
Article NumbersF5507
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection limit) The SureFast® Enterobacteriaceae PLUS real-time PCR has a limit of detection of 5 DNA copies.
Cross reactivity

Cross reactivity was observed with DNA extracts from Aeromonas species and Aggregatibacter aphrophilus.

Files
Instructions
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