Intended use:

SureFast® Hepatitis E PLUS is a real-time RT-PCR for the direct and qualitative detection of hepatitis E virus.

The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.

For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche Cobas z 480 Analyzer, Roche LightCycler® 2.02 , Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.

Specifications
Art. No. F7142
Test format 100 reactions
Detection limit The SureFast® Hepatitis E PLUS real-time RT-PCR has a limit of detection of 25 RNA copies.
Files
InstructionsGerman/English
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