SureFast® Hepatitis E PLUS
Art. No.: F7142
SureFast® Hepatitis E PLUS is a real-time RT-PCR for the direct and qualitative detection of hepatitis E virus.
The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche Cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
|For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
|LOD (Detection Limit)
|The SureFast® Hepatitis E PLUS real-time RT-PCR has a limit of detection of 25 RNA copies.