The test is a real-time RT-PCR for the direct and qualitative detection of hepatitis A virus. The test contains an Internal Control RNA (ICR) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC) at the same time (Rotor-Gene Q, Stratagene Agilent MxSeries, Roche LightCycler® 480/2.0*/1.5* etc.).
|Test format||100 reactions|
|LOD (Detection limit)||The SureFast® VIRUS Hepatitis A PLUS real-time RT-PCR has a limit of detection of ≤ 50 RNA copies. The|
assay limit of detection depends on RNA preparation and RNA content.