Intended use:

The test is a real-time RT-PCR for the direct and qualitative detection of hepatitis A virus. The test contains an Internal Control RNA (ICR) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.

For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC) at the same time (Rotor-Gene Q, Stratagene Agilent MxSeries, Roche LightCycler® 480/2.0*/1.5* etc.).

Art. No. F7125
Test format 100 reactions
LOD (Detection limit) The SureFast® VIRUS Hepatitis A PLUS real-time RT-PCR has a limit of detection of ≤ 50 RNA copies. The
assay limit of detection depends on RNA preparation and RNA content.
SDSF7125_MSDS_Hepatitis-A-PLUS_2.0.pdf (English)
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