Qualitative detection of Lupine DNA.
The detection system system can be used on all available real-time PCR cyclers (ABI Prism, Eppendorf realplex, iCycler, Rotorgene, LightCycler). For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
Lupin flour is used in food as additive to wheat flour. Lupin is also used in gluten-free bakery-products and food for celiac disease patients. The frequency of cross allergies between peanut and lupin is high. According to the EU Directive 2006/142/EC, lupin belongs to the allergenic ingredients which must be labelled. Currently there is no threshold proposed as limit up to which a product is allowed to contain lupin without indication at the label.
|Test format||100 reactions|
|Detection limit||Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to ≤ 0.4 ppm.|