The test detects lupin DNA (Lupinus spp.) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg lupin / kg food sample and a detection system including a standard dilution is required.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.|
|LOD (Detection limit)||The SureFood® ALLERGEN Lupin PCR has a limit of detection of ≤ 0.4 mg / kg.|
The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1.