Intended use:

The test detects lupin DNA (Lupinus spp.) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg lupin / kg food sample and a detection system including a standard dilution is required.

 

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.

Accessories:

Specifications
Art. No. S3611
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.
LOD (Detection limit) The SureFood® ALLERGEN Lupin PCR has a limit of detection of ≤ 0.4 mg / kg.
The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1.
Files
InstructionsGerman/English
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