Intended use:

Easy to use microbiological microtiter plate test for the quantification of total biotin (enriched and natural biotin) in food, feed and pharmaceutical products.

General information:

Biotin is extracted from the sample and the extract is diluted. The diluted extract and the biotin assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply of biotin. Following the addition of biotin as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted biotin is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).

Accessories:

Certificates:

Specifications
Art. No. P1003
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time 44 - 48 h in the dark at 37 °C (98.6 °F)
Files
InstructionsGerman/English
SDSP1003_german.zip (German)
P1003_french.zip (French)
P1003_english.zip (English)
P1003_spanish.zip (Spanish)
P1003_italian.zip (Italian)
Similar products
EASI-EXTRACT® BIOTIN
EASI-EXTRACT® BIOTIN
Immunoaffinity columns for use in conjuntion with an HPLC or LC-MS/MS system for detection of biotin in a wide range of commodities.
VitaFast® Vitamin B7 (Biotin) AOAC-RI (101001)
VitaFast® Vitamin B7 (Bioti...
Easy to use microbiological microtiter plate test for the quantification of total biotin (enriched and natural biotin) in food, feed and pharmaceut...
EASI-EXTRACT® MULTI-VIT B (LGE)
EASI-EXTRACT® MULTI-VIT B (...
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range o...

Start typing and press Enter to search