EuroProxima DNSH
Art. No.: 5091DNSH
Product info
Intended use
EuroProxima DNSH is a competitive enzyme immunoassay for the quantitative detection of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.
General Information
Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolised to 3,5-dinitrosalicyclic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatisation of this metabolite with 2-nitrobenzaldehyde to NPDNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. In accordance with Commission Regulation (EU) 2019/1871 a reference point of action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.
DNSH ELISA is a specific method for the detection of DNSH in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn). The antibody used in this test detects DNSH without derivatisation with 2-nitrobenzaldehyde.
Accessories
| Specifications | |
|---|---|
| Art. No | 5091DNSH |
| Test format | Microtiter plate with 96 wells (12 strips with 8 wells each) |
| Sample preparation | For mentioned matrices fast and efficient extraction methods are included in the kit description. |
| Incubation time | 1 h (20 - 25 °C) |
| LOD (Detection Limit) | Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg |
| Detection Capability | 0.25 µg/kg |
| Validated matrices | Meat and seafood. |
| Detected analyte | 3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Files | |
|---|---|
| Instructions | English |
| MSDS | 5091DNSH-SDS-1.zip (English) |
