The SureFood® ALLERGEN 4plex Cereals is a real-time PCR for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Agilent AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.|
|Detection limit||The SureFood® ALLERGEN 4plex Cereals PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.|