Intended use:

The SureFood® ALLERGEN 4plex Cereals is a real-time PCR for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences.

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Agilent AriaDx and Agilent Mx3005P.

Accessories:

Specifications
Art. No. S7006
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.
Detection limit The SureFood® ALLERGEN 4plex Cereals PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.
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