SureFood® ALLERGEN 4plex EU NUTS

Art. No.: S3404

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SureFood® ALLERGEN 4plex EU NUTS

Art. No.: S3404

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Intended use

The SureFood® ALLERGEN 4plex EU NUTS is a real-time PCR for the direct, qualitative detection and differentiation of

  • almond (Prunus dulcis),
  • cashew (Anacardium occidentale),
  • pistachio (Pistacia vera),
  • peanut (Arachis hypogaea),
  • hazelnut (Corylus avellana),
  • walnut (Juglans regia),
  • pecan (Carya illinoinensis),
  • brazil nut (Bertholletia excelsa) and
  • macadamia (Macadamia tetraphylla)

ccording to directive (EC) 1169/2011 in food.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.

Accessories

Specifications
Art. NoS3404
Test format100 reactions
Sample preparationFor DNA preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The detection limit is ≤ 1 mg allergenic substance / kg food (ppm) using SureFood® PREP Advanced. The LOD was determined using laboratory reference material SureFood® QUANTARD Allergen 40 (containing 40 mg/kg nuts in a corn flour matrix).
Cross Reactivity.

The pecan detection system shows 100% cross reactivity to shagbark hickory (Carya ovata).