SureFood® ALLERGEN 4plex EU NUTS

Art. No.: S3404

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SureFood® ALLERGEN 4plex EU NUTS

Art. No.: S3404

Intended use

The SureFood® ALLERGEN 4plex EU NUTS is a real-time PCR for the direct, qualitative detection and differentiation of

  • almond (Prunus dulcis),
  • cashew (Anacardium occidentale),
  • pistachio (Pistacia vera),
  • peanut (Arachis hypogaea),
  • hazelnut (Corylus avellana),
  • walnut (Juglans regia),
  • pecan (Carya illinoinensis),
  • brazil nut (Bertholletia excelsa) and
  • macadamia (Macadamia tetraphylla)

ccording to directive (EC) 1169/2011 in food.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.


Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal

Art. NoS3404
Test format100 reactions
Sample preparationFor DNA preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The detection limit is ≤ 1 mg allergenic substance / kg food (ppm) using SureFood® PREP Advanced. The LOD was determined using laboratory reference material SureFood® QUANTARD Allergen 40 (containing 40 mg/kg nuts in a corn flour matrix).
Cross Reactivity.

The pecan detection system shows 100% cross reactivity to shagbark hickory (Carya ovata).