Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A and zearalenone in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected and injected prior to analysis by LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.


  • One sample preparation and extraction for the analysis of aflatoxins, ochratoxin and zearalenone saving time.
  • Simultaneous detection of aflatoxins and ochratoxin in one HPLC run and separate detection of zearalenone in another.
  • Alternatively, analysis of all toxins can be performed in a single LC-MS/MS run. Can be used for the analysis of certain metabolites and masked mycotoxins.


Article NumbersRBRP112 / RBRP112B
Test format RBRP112: 10 immunoaffinity columns
(3 ml format),
RBRP112B: 50 immunoaffinity columns
(3 ml format)
Sample preparation A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.
Incubation time 60 seconds
LOD (Detection limit) B1 0.031 ng/ml, B2 0.015 ng/ml,
G1 0.015 ng/ml, G2 0.007 ng/ml,
OTA 0.05 ng/ml, ZON 1.67 ng/ml
Validated matrices

Food and feed.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Deviation from the methods described in our Instructions For Use and Application Notes may not result in optimum results. Please contact your local R-Biopharm distributor for further information.

Detected analyte

Aflatoxins B1, B2, G1, G2, ochratoxin A and zearalenone in food and feed.

Evaluation It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.
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