A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices.
Colistin A and B are polypeptide antibiotics that have been used in medicine and veterinary for over 50 years. The European Medicines Agency has proposed to reduce the use of the antibiotic drug colistin in food producing animals. For several food matrices maximum residue limits (MRLs) for colistin are set in the European Union: 150 μg/kg (ppb) for muscle, fat and liver; 200 μg/kg for kidney, 50 μg/l for milk; 300 μg/kg for eggs.
The Colistin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against colistin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each). |
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description. |
|Incubation time||1 h, RT|
|LOD (Detection limit)||Milk 4.3 µg/l, egg 22 µg/kg, chicken 11.7 µg/kg, pork 7.6 µg/kg, beef 14.6 µg/kg, liver 21.3 µg/kg, fish 12.5 µg/kg, feed 24.1 µg/kg |
|Detection capability CCβ||Milk 10 µg/l, egg 30 µg/kg, chicken 30 µg/kg, pork 30 µg/kg, beef 30 µg/kg, liver 30 µg/kg, fish 30 µg/kg, feed 30 µg/kg|
Milk, egg, chicken, pork, beef, liver, fish and feed.
|Detected analyte||Colistin in milk, egg, chicken, pork, beef, liver, fish and feed.|
|Evaluation||Microtiter plate spectrophotometer (450 nm) |