A competitive enzyme immunoassay for screening and quantitative analysis of colistin.
Colistin A and B are polypeptide antibiotics that have been used in medicine and veterinary for over 50 years. The European Medicines Agency has proposed to reduce the use of the antibiotic drug colistin in food producing animals. For several food matrices maximum residue limits (MRLs) for colistin are set in the European Union: 150 μg/kg for muscle, fat and liver; 200 μg/kg for kidney, 50 μg/kg for milk; 300 μg/kg for eggs.
The Colistin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against colistin.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, egg, chicken, pork, beef, liver, fish and feed fast and efficient extraction methods are included in the kit manual.|
|Incubation time||60 minutes|
|LOD (Detection limit)||Milk 4 ppb|
Eggs 22 ppb
Chicken 12 ppb
Pork 8 ppb
Beef 15 ppb
Liver 21 ppb
Fish 12 ppb
Feed 24 ppb
Colistin A and B 100%, Polymyxin B1 and B2 100%, Bacitracin <0.1%