A competitive enzyme immunoassay for quantitative analysis of okadaic acid in scallop, mussel and oyster samples.
High concentrations of shellfish toxins can accumulate in filter feeders such as clams, mussels, and oysters causing illness amongst people who eat them. There are four syndromes called shellfish poisoning, i.e paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP). DSP is primarily caused by the polyether toxin okadaic acid (OA) and several analogues of OA, among which the dinophysistoxins DTX1, DTX2, and DTX3.
In the European Union, Regulation (EC) no 853/2004 stipulates that live bivalve mollusks must not contain OA in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 160 μg OA equivalents (OA, dinophysistoxins, and pectenotoxins together) per kilogram.
The Okadaic Acid ELISA is a competitive enzyme immunoassay based on antibodies directed against okadaic acid.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For mussel and oyster fast and efficient methods are included in the kit manual.|
|Incubation time||45 minutes|
|LOD (Detection limit)||Mussel 40 CCβ|
Oyster 40 CCβ
Okadaic acid 100% Dinophysistoxins DTX-1 78%, Dinophysistoxins DTX-2 2.6%