A competitive enzyme immunoassay for quantitative analysis of saxitoxin in scallop, mussel and oyster samples.
High concentrations of shellfish toxins can accumulate in filter feeders such as clams, mussels, and oysters causing illness amongst people who eat them. There are four syndromes called shellfish poisoning, i.e paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP). PSP is caused by a wide number of derivatives of saxitoxin (STX).
In the European Union, Regulation (EC) no 853/2004 stipulates that live bivalve mollusks must not contain PSP toxins (saxitoxin and analogues) in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 800 μg per kilogram.
The Saxitoxin ELISA is a competitive enzyme immunoassay based on antibodies directed against saxitoxin.
|Test format||Microtiter plate, 96 wells|
|Sample preparation||For mussel and oyster fast and efficient methods are included in the kit manual.|
|Incubation time||45 minutes|
|LOD (Detection limit)||Mussel 4 CCβ|
Oyster 3 CCβ
Saxitoxin 100%, Decarbamoyl saxitoxin 19.2%, Gonyautoxins, GTX 2/3 5.6%, GTX 5 26.2%, Decarbamoyl GTX 2/3 0.2%, Neosaxitoxin 1.4%, Decarbamoyl neosaxitoxin 0.5%, Gonyautoxins GTX 1/4 < 0.1%, C 1/2 0.2%