A competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples.
High concentrations of shellfish toxins can accumulate in filter feeders such as clams, mussels, and oysters causing illness amongst people who eat them. There are four syndromes called shellfish poisoning, i.e paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP). ASP is caused by domoic acid (DA), an excitatory amino acid containing the structure of glutamic acid and resembling kainic acid. In the European Union, Regulation (EC) no 853/2004 stipulates that live bivalve mollusks must not contain DA in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 20 mg DA per kilogram.
The Domoic acid ELISA is a competitive enzyme immunoassay based on antibodies directed against domoic acid.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For scallop, mussel and oyster fast and efficient methods are included in the kit manual.|
|Incubation time||45 minutes|
|LOD (Detection limit)||Scallop 60 CCβ|
Mussel 60 CCβ
Oyster 150 CCβ
Domoic acid 100%, Okadaic acid < 0.10%, DTX-1 and DTX-2 < 0.10%, Saxitoxin < 0.10%, Neosaxitoxin < 0.10%, Gonyautoxins GTX 2/3, 1/4 < 0.10%, B1 and B2 < 0.10%, C 1/2 < 0.10%