A competitive enzyme immunoassay for screening and quantitative analysis of several promazines in various matrices.
Promazines belong to the phenothiazine class of antipsychotics. These agents are tranquillisers that are commonly used to reduce stress during transportation of food producing animals. Furthermore, promazines are used in treating restlessness and agitation of horses and domestic cattle. In the EU the use of phenothiazine derivatives (chlorpromazine, acepromazine, propionylpromazine) to treat animals intended for human consumption is totally prohibited. There exists no Maximum Residue Limits (MRLs) for these agents.
The Promazine, generic ELISA is a competitive enzyme immunoassay based on antibodies directed against promazine.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For urine, tissue, liver and kidney fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|Detection limit||Urine 1.1 ppb|
Tissue 4.3 ppb
Liver 0.2 ppb
Kidney 0.3 ppb
Propionyl promazine 130% Chlorprothixene 20%, Acepromazine 110% Fluphenazine <0.01%, Chlorpromazine 100% Xylazine <0.01%, Promazine 50%